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Table 3 Performance of the wild-type P . putida KT2440 and the streamlined strain EM383 as a host for recombinant DNA

From: Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

P. putida strain

Plasmid transfer efficiency (transformants per 1 × 10 9cells)

Mating a

Electroporation b

KT2440

(5 ± 1) × 106

5,483 ± 3,060

EM383

(18 ± 5) × 106

1,642 ± 822

  1. aThe plasmid transfer efficiency by tri-parental mating was calculated using vector pSEVA221, and the figures represent the mean value and SD of three biological replicates.
  2. bThe plasmid transfer efficiency by electroporation was calculated using vector pSEVA251, and the figures represent the mean value and SD of three biological replicates when using 1 ng of vector.
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Microbial Cell Factories

ISSN: 1475-2859

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