Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

Table 2 Growth characterization of the wild-type P . putida KT2440 and the streamlined strain EM383 using different C sources

P . putida strain	Specific growth rate ^a(h ⁻¹) in:
	LB medium	M9 minimal medium amended with
	LB medium	Succinate	Citrate	Glucose	Fructose
KT2440	0.74 ± 0.01	0.44 ± 0.05	0.46 ± 0.08	0.32 ± 0.02	0.20 ± 0.01
EM383	0.56 ± 0.08	0.41 ± 0.05	0.39 ± 0.04	0.40 ± 0.1	0.22 ± 0.01

^aThe specific growth rate for each strain was calculated during exponential growth. The cultures were carried out in 96-well microtiter plates, and the optical density at 600 nm was measured every 15 min during 24 h using a SpectraMax M2e microplate reader. All tested C sources were added to M9 minimal medium at 0.2% (w/v). Results represent the mean and SD of three independent experiments.

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