Figure 4

Characterization of the energy and redox status of wild-type P . putida KT2440 and the streamlined strain EM383. (A) The ATP content on biomass (Y _ATP/X) and the adenylate energy charge (AEC) were calculated for exponentially-growing cells in shaken-flask cultures using M9 minimal medium containing 0.2% (w/v) glucose. Each bar represents the mean value and SD of the ATP content on biomass (CDW, cell dry weight) or the adenylate energy charge for duplicate measurements from at least three independent experiments. (B) Redox ratios were determined from the absolute intracellular concentrations of NAD⁺, NADH, NADP⁺, and NADPH. The pyridine nucleotide cofactors were enzymatically determined in exponentially-growing cells in shaken-flask cultures using M9 minimal medium containing 0.2% (w/v) glucose. Bars represent mean values along with SD of the corresponding parameter for duplicate measurements from at least three independent experiments. The asterisk (*) indicates a significant difference for strain EM383 as compared to wild-type KT2440 according to the Student’s t test (P <0.05).