Figure 2

Growth parameters of P . putida KT2440 and the streamlined strain EM383. (A) Duration of the lag phase of wild-type KT2440 cells (blue) and the streamlined strain EM383 (green) in rich LB medium or M9 minimal medium added with 0.2% (w/v) of either succinate (Suc), citrate (Cit), glucose (Glu), or fructose (Fru). The extent of the lag phase was calculated using data from growth curves as described by Dalgaard and Koutsoumanis [67]. (B) Final cell density (estimated as the optical density at 600 nm, OD₆₀₀) of shaken-flask cultures of wild-type KT2440 (blue) and the streamlined strain EM383 (green) in rich LB medium. (C) Final cell density (estimated as the OD₆₀₀) of shaken-flask cultures of wild-type KT2440 (blue) and the streamlined strain EM383 (green) in M9 minimal medium added with 0.2% (w/v) of either succinate (Suc), citrate (Cit), glucose (Glu), or fructose (Fru). In all cases, the mean values of the corresponding parameter are plotted along with the SD of three independent experiments. The asterisks indicate a significant difference in the corresponding parameter when comparing strain EM383 and wild-type KT2440 according to the Student’s t test (*, P <0.05; and **, P <0.01).