Table 2 Primers used in this study
Name | Sequence (5′-3′) (restriction site with italic formatting) | Use |
|---|---|---|
7301-P1 | CGCGAATTCGCATCCTCG AGCACTTCACCG (EcoRI) | Inactivation of SACE_7301 |
7301-P2 | CCCGGTACCCGGCTCCGGATGGAACCG (KpnI) | |
7301-P3 | CTCTCTAGACACCACGGCGGCCTGCCG GC (XbaI) | |
7301-P4 | CCAAAGCTTGGAGTCGGCGCATGCTGGTCTC (HindIII) | |
7301-P5 | AGACATATGATGAAGGCCGACGTGGAGCAC (NdeI) | Complementation and overexpression of SACE_7301 |
7301-P6 | GCAGATATCTCACTCCGGTTT CCAGTCGCG (EcoRV) | |
apr-F | GCTCATCGGTCAGCTTCTCA | |
apr-R | TCGCATTCTTCGCATCCC | |
7301-P7 | GCTGGGTGTACTCGAAGAACGA | qRT-PCR analysis of SACE_7301 |
7301-P8 | TGGGGTCGAAGGAGGAGC | |
7301-P9 | AGACATATGATGAAAGCCGATGTGGAGCATTCTGATCGTCCGCGTCCGCGTACCAAGCGGCTGCCGCGC (NdeI) | Expression of SACE_7301 In E. coli |
7301-P10 | CCCAAGCTTCTCCGGTTTCCAGTCGCGGCC (HindIII) | |
bldD-F | AAACATATGATGGGCGACTACGCCAAGGCGCTGGG (NdeI) | Expression of BldD In E. coli |
bldD-R | TGTAAGCTTCTCCTCCCGGGCCGGGCGC (HindIII) | |
ermE-F | TAATCTAGAGCGAGTGTCCGTTCGAGTGG (XbaI) | Cloning of combined DNA fragment containing PermE* and SACE_7301 |
7301-R | GCAACTAGTTCACTCCGGTTTCCAGTCGCG (SpeI) | |
eryAI-P1 | CCGCTGATGCCGAACGAC | qRT-PCR analysis of eryAI |
eryAI-P2 | CACCCTTCCCCGCACTCTG | |
eryAI-P3 | CGGAGCATTTGCTCGCTTTCCAGG | EMSA of eryAI promoter |
eryAI-P4 | GCGTCCCCCTACTCGACGACCAC | |
ermE-P1 | CCTCCAGGCACCAGTCCAC | qRT-PCR analysis of ermE |
ermE-P2 | AGTCGTTGCGGGAGAAGCT | |
ermE-P3 | GCGAGTGTCCGTTCGAGTGGCGG | EMSA of ermE promoter |
ermE-P4 | CGCTGGATCCTACCAACCGGCAC | |
hrdB-F | GGTCACGCCGTAGACCTGGC | qRT-PCR analysis of hrdB as internal reference |
hrdB-R | CGGTGTCGTTCACGCTGCTG | |
Test-F | GCCAGTGCCAAGCTTGGGCTGCAGGTCGAC | PCR analysis of the cassette containing 3 copies of PermE*-SACE_7301 |
Test-R | GAATTCGATATCGCGCGCGGCCGCGGATCC |