Figure 3From: Dissecting and engineering of the TetR family regulator SACE_7301 for enhanced erythromycin production in Saccharopolyspora erythraea Electrophoretic mobility shift assays (EMSAs) with the promoter regions of eryAI or ermE and purified His 6 -tagged SACE_7301 or His 6 -tagged BldD. (A) EMSAs with recombinant SACE_7301 protein and PeryAI. (B) EMSAs with recombinant SACE_7301 protein and PermE. (C) EMSAs with recombinant BldD protein and PeryAI. (D) EMSAs with recombinant BldD protein and PermE. Each of the lanes contained 10 ng DNA. Results were one representative example of at least three replicates.Back to article page