Number of rec Sodalis CFU (DNA equivalent) in abdomen and thorax of streptozotocin-treated and non-treated male flies injected with 5x10 4
CFU of Sod _pFliCpelBNb46 fliC . Prior to injection, treated flies were given three blood meals supplemented with 20 μg/ml streptozotocin during the first week after emergence, while non-treated flies received normal blood meals. qPCR on the pFliCpelBNb46FliC plasmid was used as a means to estimate the number of recSodalis. The bars represent the mean recSodalis CFU (DNA equivalent) ± SD present in abdomen and thorax tissues of at least 5 individual flies from each treatment group at the time of sampling. The number of CFU (DNA equivalent) is represented in log scale on the y-axis. P values were calculated using the Mann–Whitney U test (*p <0.05, **p <0.01).