In vitro characteristics of rec Sodalis expressing Nb_An46. A) Growth curve analysis of WT Sodalis and Sod_pFliCpelBNb46fliC. The error bars show the ± SD of two biological replicates. Samples were taken every 24 h. B) ELISA-based Nb_An46 quantitation (bar-chart) of the intra- and extracellular nanobody concentration produced by Sod_pFliCpelBNb46fliC at selected time points in relation to bacterial cell density (OD600nm, solid line) using a 6 × His tag specific detection antibody. Values are presented as ng recombinant protein per ml culture medium.