Figure 3

Influence of commercial cellulolytic enzyme cocktails in yeast ( P. aphidis ) growth and viability in 40 g/l glucose (carbon source). (a) Yeast growth in the presence of Celluclast 1.5 L^® loading: medium, 1.75% v/v (triangles), medium-high, 3.50% v/v, (squares), high, 5.25% v/v (crosses); (b) Yeast growth in the presence of Novozyme 188^® loading: medium, 0.25% v/v (triangles), medium-high, 0.50% v/v (squares), high 0.75% v/v (crosses); (c) Viable yeast cells after 96 h-culture in the presence of: medium (1.75% v/v), medium-high (3.5% v/v) and high (5.25% v/v) Celluclast 1.5 L^® loading (A); and medium (0.25% v/v), medium-high, (0.50% v/v) and high (0.75% v/v) Novozyme 188^® loading (B); (d) Yeast growth in the presence of Celluclast 1.5 L^®, 5.25% v/v plus Novozyme 188®, 0.75% v/v (black lines): active enzymes (filled diamonds), inactivated enzymes (open diamonds). Control (glucose without enzymes) (dashed grey line, open diamonds).