Skip to main content
Figure 2 | Microbial Cell Factories

Figure 2

From: Conversion of cellulosic materials into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma spp. under SHF and SSF processes

Figure 2

MEL production from commercial grade cellulose (Avicel ® ) by P. antarctica PYCC 5048 T and P. aphidis PYCC 5535 T by SSF. SSF was carried out with 40 g/l glucan at 28°C and 140 rpm, supplemented with low enzyme loading. (a) Time course of SSF showing glucose concentration (squares) and MEL formation (triangles) with P. antarctica (continuous line) and P. aphidis (dashed line). (b) Thin Layer Chromatography (TLC) of MEL obtained from liquid-liquid extraction with ethyl acetate of culture broth samples (after 10 days of SSF). Samples were eluted with the solvent system of CHCl3/CH3OH/NH4OH (65:15:2) to separate MEL-A, -B, -C and -D, which were visualized after reaction of α-naphthol reagent with the sugar moiety of MEL. Lines: 1 and 4 - MEL standard (MEL-A, -B, -C and -D [19]); 2 - P. antarctica SSF; 3 - P. aphidis SSF.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us