Figure 7From: Genetic and biochemical approaches towards unravelling the degradation of gallotannins by Streptococcus gallolyticus Some biochemical properties of TanA Sg protein. (A) Relative activity versus pH. (B) Relative activity of TanASg versus temperature. (C) Thermal stability of TanASg after preincubation at 22°C (filled diamond), 30°C (filled square), 37°C (filled triangle), 45°C (cross), 55°C (star), and 65°C (filled circle) in phosphate buffer (50 mM, pH 6.5); at indicated times, aliquots were withdrawn, and analyzed as described in the Methods section. The observed maximum activity was defined as 100%. (D) Relative activity of TanASg after incubation with 1 mM concentrations of different additives. The activity of the enzyme in the absence of additives was defined as 100%. The experiments were done in triplicate. The mean value and the standard error are shown. Asteriks indicate a p value <0.05.Back to article page