Figure 6
Tannase activity of S. gallolyticus TanA Sg protein against simple phenolic esters (1) and complex tannins (2). Hydrolase activity of purified TanASg protein was analyzed on triplicate and compared with control reactions on which the enzyme was omitted. HPLC chromatograms of TanASg (100 μg) incubated in 1 mM of methyl gallate (A), ethyl gallate (B), propyl gallate (C), ethyl protocatechuate (D), epigallocatechin gallate (E), and tannic acid (F). The methyl gallate (MG), ethyl gallate (EG), propyl gallate (PG), ethyl protocatechuate (EP), epigallocatechin gallate (EGCG), tannic acid (TA), gallic acid (GA), protocatechuic acid (PA), and epigallocatechin (EGC) detected are indicated. The chromatograms were recorded at 280 nm.