Figure 5
SDS-PAGE analysis of the expression and purification of S. gallolyticus TanA Sg protein. Analysis of soluble cell extracts of IPTG-induced E. coli BL21(DE3) (pURI3-TEV) (1) or E. coli BL21(DE3) (pURI3-TEV-TanASg) (2), flowtrough (3), or fractions eluted after His affinity resin (4-8). The arrow indicated the overproduced and purified protein. The gel was stained with Coomassie blue. Molecular mass markers are located at the left (SDS-PAGE Standards, Bio-Rad).