Table 2 Recombinant protein production in bioreactor and SSB system

GFP

Bioreactor (2-L) _{constant agitation (500 rpm)}

17.6 ± 0.5

6.8

8

9

15420 ± 120

857 ± 25

SSB system (2-L)

20.6 ± 0.3

5.9

8

9

18172 ± 140

865 ± 14

hFGF-2

Bioreactor (2-L) _{constant agitation (500 rpm)}

18.8 ± 0.5

6.8

9

8

Bioreactor (30-L) _{dissolved oxygen-stat (50%)} ^#

18.0 ± 0.6

6.8

8

7

SSB system (2-L)

18.9 ± 0.3

5.9

9

7

SSB system (2-L)

21.0 ± 0.4

6.0

9

8

GST-GFP

Bioreactor (2-L) _{constant agitation (500 rpm)}

22.3 ± 0.1

6.8

10

7

11569 ± 163

526 ± 8

SSB system (2-L)

24.7 ± 0.2

5.8

10

6

13044 ± 102

522 ± 6

SSB system (2-L) with “booster” amino acids

26.2 ± 0.2

6.5

10

9

17981 ± 158

692 ± 8

TRX-hLIF

Bioreactor (2-L) _{constant agitation (500 rpm)}

16.0 ± 0.2

6.8

7

1

Bioreactor (2-L) _{dissolved oxygen-stat (30%)}

18.9 ± 0.2

6.8

8, 7^*

2, 3^*

SSB system (2-L)

19.0 ± 0.3

5.9

7

1

SSB system (2-L)

18.9 ± 0.3

6.0

7

2

SSB system (2-L) with “booster” amino acids

23.2 ± 0.4

6.6

7, 7^*

3, 7^*

SSB system (2-L) with “booster” amino acids

22.6 ± 0.1

6.5

8

3