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Figure 3 | Microbial Cell Factories

Figure 3

From: Smart sustainable bottle (SSB) system for E. coli based recombinant protein production

Figure 3

Production of hFGF-2 in a conventional 2-L bioreactor and the SSB system. Autoinduction cultivations were carried out in a 2-L bioreactor (A1-A4) and the SSB system (B1-B4) for the production of hFGF-2. Carbon dioxide (CTR, black line) and oxygen transfer rates (OTR, gray line), dissolved oxygen concentration (light gray line), and cell growth (squares) are shown (A1, B1). Moreover, the consumption of glucose (black down triangle), lactose (dark gray up triangle), and glycerol (black circle) as well as the production of acetate (gray diamond) are given (A2, B2). hFGF-2 production in the bioreactor and SSB system was analyzed by SDS-PAGE. W: whole cell protein, S: soluble, and I: insoluble part of whole cell protein (A3, B3). Kinetics of product formation were analyzed by SDS-PAGE of the whole cell protein (A4, B4).

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