Figure 2
From: Smart sustainable bottle (SSB) system for E. coli based recombinant protein production
Production of GFP in a conventional 2-L bioreactor and the SSB system. Autoinduction cultivations were carried out in a 2-L bioreactor (A) and the SSB system (B) for the production of GFP. Carbon dioxide (CTR, black line) and oxygen transfer rates (OTR, gray line), and dissolved oxygen concentration (light gray line) are shown. GFP production in the bioreactor (lanes 1-3) and SSB system (lanes 4-6) was analyzed by SDS-PAGE. W: whole cell protein, S: soluble, and I: insoluble part of whole cell protein (C).