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Figure 8 | Microbial Cell Factories

Figure 8

From: OpenFLUX2: 13C-MFA modeling software package adjusted for the comprehensive analysis of single and parallel labeling experiments

Figure 8

Normalized flux precision functions for all fluxes are presented in the form of color-scored squared diagrams for the different tracers used for simulations. The columns correspond to the following labeling experiments: 1, 2, …., 6 − SLEs with 100% [i-13C]-glucose as a tracer, where i = 1, 2, …, 6, respectively; 7 − an SLE with a generally optimized (minimum D-value) mixture of [U-13C]65%/[U-12C]35%-glucose; 8 − a PLE consisting of 5 LEs with different mixtures of [U-13C]/[U-12C]-glucose in each LE, where the fraction of [U-13C]-glucose was 20%, 35%, 50%, 65%, or 80%; 9 − a PLE consisting of 5 LEs with partially optimized mixtures of [1-13C]/[U-13C]/[U-12C] glucose for separate minimization of the approximated variances of the θ 10, θ 12, θ 14, θ 22, θ 31 free fluxes in each LE; 10 − a PLE composed of 3 LEs using 100% [1-13C]-, [3-13C]-, or [4-13C]-glucose as the tracer; 11 − a PLE consisting of 6 LEs, where all singly labeled isotopomers of glucose, as in 1 – 6, were used as tracers; 12 − a PLE composed of 6 LEs, including 3 of the LEs used in 10, corresponding to partial optimization for θ 4 and θ 12, θ 14, θ 22andθ 31, and 3 other LEs using the partially optimized mixture of [1-13C]/[3-13C]/[4-13C]-glucose to minimize the approximated variances of the θ 8, θ 10, θ 26 free fluxes.

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