Figure 3

PCA analysis of GC-MS metabolomic profiles. A) Plot of the experiment I (S0, S1, S3 and S4 cells from day 0, 28, 205 and 350 of the evolution course, corresponding to their maximal butanol tolerance of 0.2, 0.2, 0.35 and 0.5%, respectively) grown in media supplemented with 0.2% (v/v) butanol; B) Plot of the experiment II (cells from day 0, 28, 205 and 350 of the evolution course, corresponding to their maximal butanol tolerance of 0.2, 0.2, 0.35 and 0.5%, respectively) grown in media supplemented with butanol equal to their maximal butanol tolerance levels, respectively; C) Loading plot of the experiment I; D) Loading plot of the experiment II.