Table 3 List of primers used in this study
From: Tuning constitutive recombinant gene expression in Lactobacillus plantarum
Primer | Sequence 5′->3′ |
|---|---|
mCherry_F (NdeI) | CAGCAGCAGCATATGTTATCAAAGGGTGAAGAAG |
mCherry_R (BamHI) | CGTCGTGGATCCTTATCACTTGTATAATTCATCCATACC |
P11_F (SacI,KpnI) | GACGACGAGCTCGGTACCTTACAGCTCCAGATCTAGCG |
P11_R (NdeI) | GACGACGACCATATGTAAAATCTCCTTGTAATAGTATTT |
sCAT_R (KpnI) | GCTGCTGGTACCGGGCAGGTTAGTGACATTAG |
Tldh_F (BamHI) | CTGCTGGGATCCAAAACCGCTGT |
mCherry_F (BsrGI) | CAGCAGTGTACAAGGAGATTTTACATATGTTATCAAAGGGTGAAG |
Ptuf_CD033_F (KpnI) | GACGACGGTACCAATTCAGCAATTTAGCCTTGATTTAC |
Ptuf33_R (BsrGI) | GTCCAGTGTACAGATTTTCGCAAGAATAACGTCG |
Ptuf_CD034_F (KpnI) | GTCGTCGGTACCGATCAGGAAATTAAAATTGGTCTC |
Ptuf34_R (BsrGI) | GTCGTCTGTACATGTTTTCAAGAATTACATCAATTTCG |
efp-sense_F (MfeI, KpnI) | GCAGCACAATTGGGTACCAATAGTTTAATTAAAGAGGCTGG |
Pefp_R (BsrGI) | CAGCAGTGTACAAAATGTAATGTAACA |
Cat_F (NheI) | CGACGAGCTAGCAATGTGGTCTTTATTCTTCAAC |
M13_R (NheI) | CGACGAGCTAGCAGCCAGGAAACAGCTATGACC |
Tldh_amp_R (PstI) | CTGCTGCTGCAGAAAAAGATTAAAAAAGCCGCTGC |
P11_control_R (KpnI) | CTGCACGGTACCCAAGGAGATTTTACATATGTTATCA |
Cat_seq2_back | TACATCATTCTGTTTGTGATGG |
4_6_n2_R | AACTCATAATACGCCTAAGCC |
EFP_screen_back | GATTCCCGATAACAACCGT |
SDOPT_5_F (XbaI) | ACGACGTCTAGATAAGGAGGAATACATGTTATCAAAGGGTGAAGAAG |
SDOPT_6_F (XbaI) | ACGACGTCTAGATAAGGAGGAAATACATGTTATCAAAGGGTGAAGAAG |
SDOPT_7_F (XbaI) | ACGACGTCTAGATAAGGAGGAAATTACATGTTATCAAAGGGTGAAGAAG |
SDOPT_8_F (XbaI) | ACGACGTCTAGATAAGGAGGAAATTTACATGTTATCAAAGGGTGAAGAAG |
SDOPT_9_F (XbaI) | ACGACGTCTAGATAAGGAGGAAATTATACATGTTATCAAAGGGTGAAGAAG |
SDOPT_10_F (XbaI) | ACGACGTCTAGATAAGGAGGAAAATTATACATGTTATCAAAGGGTGAAGAAG |
SDOPT_11_F (XbaI) | ACGACGTCTAGATAAGGAGGAAAAATTATACATGTTATCAAAGGGTGAAGAAG |
SDOPT_12_F (XbaI) | ACGACGTCTAGATAAGGAGGAAAAAATTATACATGTTATCAAAGGGTGAAGAAG |
SDOPT_R (XbaI) | ACGACGTCTAGAGAATACATATATGCTGGCCAGC |