Figure 5

Comparison of oxygen transfer rates of all E. coli variants in selected auto-induction media. Oxygen transfer rate during cultivation of E. coli BL21 (DE3) variants expressing different recombinant target proteins (3-hydroxybutyryl-CoA dehydrogenase (HBD), glucose 1-dehydrogenase (GDH), HBD-GDH, and alcohol dehydrogenase A (ADH-A)) in Overnight Express auto-induction medium ((A) OnEx_1 and (B) OnEx_2) and (C) auto-inducing TB+lactose medium (Roth_4). The respiration behavior is categorized into two patterns, as described in the caption of Figure 4. Conditions: 250-mL flask, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm, and 37°C.