Figure 3

Reproducibility of RAMOS cultivations in auto-induction media. Oxygen transfer rate during independent cultivations of E. coli BL21 (DE3) variants expressing (A-C) 3-hydroxybutyryl-CoA dehydrogenase (E. coli HBD) and (D-F) glucose 1-dehydrogenase (E. coli GDH) in two auto-inducing TB+lactose media with different yeast extracts (DSM and Roth_3) and one Overnight Express auto-induction medium (OnEx_2) as specified in Table 1. Conditions: 250-mL flask, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm, and 37°C.