Figure 9

Batch lactic acid production in wt and sam2Δ strains. Fermentation profiles for CEN.PK m850 (filled symbols) and CEN.PK m850 sam2Δ (open symbols) pre-grown in shake flasks and then transferred in new flasks in minimal medium containing 90g/L of glucose for the lactic acid production phase. (A) Biomass formation (OD at 660nm). (B) Residual glucose (diamonds) and produced lactic acid (triangles). (C) Cellular viability was determined by PI staining followed by flow cytometry. (D) Culture medium pH. Panel A and B report the mean and SD for three independent experiments (*p ≤ 0.01; Students t-test; for supplementary details see Additional file 2: Table S1); Panel C and D report data from a single representative experiment.