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Figure 7 | Microbial Cell Factories

Figure 7

From: Changes in SAM2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae

Figure 7

Viability determination for cells stressed with lactic lacid. Cells were grown in minimal medium until the exponential phase and then treated with a pulse of lactic acid. After 30min of incubation, cells were collected and stained with propidium iodide (PI) to detect dead and/or severely damaged cells by flow cytometry. The fluorescence emission was measured through a 670nm long pass filter (FL3 parameter). For each sample, 25000 cells were analysed. The bar indicates the PI positive subpopulation. Panel A: BY4741 and BY4741 sam2Δ. Panel B: BY4741 [pTEF-L], BY4741 sam2Δ [pTEF-L] and BY4741 [pTEF-L-SAM2].

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