Figure 7From: Changes in SAM2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae Viability determination for cells stressed with lactic lacid. Cells were grown in minimal medium until the exponential phase and then treated with a pulse of lactic acid. After 30min of incubation, cells were collected and stained with propidium iodide (PI) to detect dead and/or severely damaged cells by flow cytometry. The fluorescence emission was measured through a 670nm long pass filter (FL3 parameter). For each sample, 25000 cells were analysed. The bar indicates the PI positive subpopulation. Panel A: BY4741 and BY4741 sam2Δ. Panel B: BY4741 [pTEF-L], BY4741 sam2Δ [pTEF-L] and BY4741 [pTEF-L-SAM2].Back to article page