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Figure 5 | Microbial Cell Factories

Figure 5

From: Changes in SAM2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae

Figure 5

Growth of wild type and sam2Δ LEU2 genetically complemented strains in the absence and presence of lactic acid. Yeast cells were grown in shake flasks in minimal (YNB) medium with 2%w/v glucose and 50mg/L of the necessary nutritional supplements at initial pH3, without (panel A) or with (panel B) 40g/l of lactic acid. Growth was determined as OD at 660nm. Left panels: CEN.PK cells; right panels: BY cells. Dark grey squares: wild type control strains (CEN.PK 102-3A [pTEF-L], BY4741 [pTEF-L]). White triangles: sam2Δ cells (CEN.PK 102-3A sam2Δ [pTEF-L], BY4741 sam2Δ [pTEF-L]).

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