Figure 5From: Changes in SAM2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae Growth of wild type and sam2Δ LEU2 genetically complemented strains in the absence and presence of lactic acid. Yeast cells were grown in shake flasks in minimal (YNB) medium with 2%w/v glucose and 50mg/L of the necessary nutritional supplements at initial pH3, without (panel A) or with (panel B) 40g/l of lactic acid. Growth was determined as OD at 660nm. Left panels: CEN.PK cells; right panels: BY cells. Dark grey squares: wild type control strains (CEN.PK 102-3A [pTEF-L], BY4741 [pTEF-L]). White triangles: sam2Δ cells (CEN.PK 102-3A sam2Δ [pTEF-L], BY4741 sam2Δ [pTEF-L]).Back to article page