Figure 1

Western blot analysis of total Sam2p levels in cells grown in the absence and presence of lactic acid. CEN.PK 113-11C (panel A) and BY4741 SAM2 GFP (panel B) cells were grown in shake flasks in minimal (YNB) medium with 2%w/v glucose without or with the addition of different concentrations of lactic acid (pH5, pH3, 12g/L and 20g/L lactic acid at pH3) and the Sam2p-GFP levels were evaluated after 16 and 40hours after inoculation in the total protein fraction, extracted with TCA, using an anti-GFP antibody. Samples were normalised according to cell number. β-actin levels have been detected as control. Bands have been quantified by ImageJ 1.48 software. Histograms refer to the ratio (%) of Sam2p/Actin normalized to the values at pH 5. LA: lactic acid.