Figure 5

Effects of immobilization of cells on the conversion of D-sorbitol in a 1-L fermenter. A. Batch fermentation with immobilized wild-type strains and G. oxydans-sldhAB6 cells. Sqares: wild-type strain, circles: G. oxydans-sldhAB, triangles: G. oxydans-sldhAB6; B. Fed-batch fermentation with immobilized wild-type strains. Open squares: D-sorbitol, filled squares: L-sorbose; C. Fed-batch fermentation with immobilized G. oxydans-sldhAB6 cells. Open squares: D-sorbitol, filled squares: L-sorbose. 0.5 L of initial medium contained 75 g of D-sorbitol, T = 30°C, bead diameter = 2.0 mm, initial pH = 5.1, 2.5% sodium alginate, 0.5% diatomite, 0.25 L of new medium was fed and 0.25 L fermentation broth was discharged every 24 h. Error bars: Standard deviation (SD) (n = 3).