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Table 2 Enzymatic activities of recombinant S. cerevisiae strains

From: Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiaestrains for ethanol production from undetoxified lignocellulosic hemicelluloses

Strain

Specific activity

 

XR [U/mg-protein]

XDH [U/mg-protein]

TAL [U/mg-protein]

FDH [U/mg-protein]

Endoxylanase [U/g-DCW]

Xylosidase [U/g-DCW]

BGL [U/g-DCW]

Sun49-1

0.519 ± 0.033

0.979 ± 0.070

0.130 ± 0.015

0.004 ± 0.001

N.D.

N.D.

N.D.

Sun49-5

0.634 ± 0.014

1.210 ± 0.067

0.146 ± 0.022

0.004 ± 0.001

19.5 ± 2.6

94.5 ± 33.6

75.9 ± 15.8

Sun49-7

0.554 ± 0.004

1.015 ± 0.029

0.517 ± 0.018

0.011 ± 0.001

19.0 ± 3.7

86.7 ± 31.7

74.3 ± 0.7

Sun49-24

0.620 ± 0.122

1.124 ± 0.119

0.385 ± 0.010

0.008 ± 0.001

N.D.

N.D.

N.D.

  1. Values are the averages of three independent experiments, ±SEM. N.D., not detected.
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Microbial Cell Factories

ISSN: 1475-2859

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