Gene/Antibiotic | Primer | Primer sequence (5’-3’) | PCR profile (30 cycles each) | Reference |
---|---|---|---|---|
16S rRNA | fD1rD1 | AGAGTTTGATCCTGGCTCAG | Denaturing: 95’C 2 min. | [51] |
Annealing: 55’C 30 sec. | ||||
AAGGAGGTGATCCAGCC | ||||
Extension: 72 °C 5’min. | ||||
Surfactin | Sfp-f | ATG AAG ATTTACGGAATTTA | Denaturing: 94 °C 1 min. | [52] |
Annealing: 48 °C 1 min. | ||||
Sfp-r | TTATAAAAGCTCTTCGTACG | |||
Extension: 72 °C 1 min. | ||||
Iturin A | ituD-f | ATG AAC ATCTTGCCTTTTTA | Denaturing: 94 °C 1 min. | [53] |
Annealing: 50 °C 1 min. | ||||
ituD-r | TTATTTTAAAATCCGCAATT | |||
Extension: 72 °C 1.5’min. | ||||
TRFLP-16S rDNA | VIC-63’F | CAGGCCTAACACATGCAAGTC | Denaturing: 94 °C 30 Sec. | |
Annealing: 56 °C 30 Sec. | ||||
FAM-1389R | ACGGGCGGTGTGTACAAG | |||
Extension: 72 °C 2 min. | ||||
TRFLP-Fungal ITS | FAM -ITS1 | TCCGTAGGTGAACCTTGCGG | Denaturing: 94 °C 30 Sec. | [55] |
Annealing: 55’C 30 Sec. | ||||
VIC- ITS4 | TCCTCCGCTTATTGATATGC | |||
Extension: 72 °C 30 Sec. |