Using ionic liquids in whole-cell biocatalysis for the nucleoside acylation

Table 2 Effect of different organic cosolvents on acylation of ara-C by P. fluorescens in IL-containing systems ^a

Entry	IL-containing system	logPvalue^e	V₀(mmol/L h)	Y(%)	3′-regioselectivity (%)	5′-regioselectivity (%)
1	[OMI][BF₄]/acetone^b,d	-0.23	0.74	9.4	10.5	89.5
2	[OMI][BF₄]/THF^b,d	0.49	0.77	32.1	<1.0	>99.0
3	[OMI][BF₄]/t-butanol^b,d	0.80	1.06	13.5	8.25	91.8
4	[OMI][BF₄]/Isopropyl ether^b,d	1.90	0.80	12.9	<1.0	>99.0
5	[OMI][BF₄]/hexane^b,d	3.50	1.11	14.4	<1.0	>99.0
6	[BMI][PF₆]/acetone^c,d	-0.23	1.56	26.1	<1.0	>99.0
7	[BMI][PF₆]/THF^c,d	0.49	2.34	81.1	<1.0	>99.0
8	[BMI][PF₆]/t-butanol^c,d	0.80	1.98	18.8	<1.0	>99.0
9	[BMI][PF₆]/Isopropyl ether^c,d	1.90	0.64	9.0	<1.0	>99.0
10	[BMI][PF₆]/hexane^c,d	3.50	0.37	5.3	<1.0	>99.0

^aThe reactions conditions: 50 mg/mL biomass, 20 mmol/L ara-C, 900 mmol/L VL, 40 μL water, 1 mL reaction media, 40°C, 200 rpm and 144 h. ^b[OMI][BF₄] content, 80% (v/v); ^c[BMI][PF₆] content, 10% (v/v); ^d10% Pyridine (v/v) was added as an assistant solubilizer for dissolving ara-C. ^elogP value of the organic cosolvents, referred to Ref. (Trends in Biotechnology, 1995, 13(2):63-70).

ISSN: 1475-2859