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Table 1 Yield comparison of anti-HER2 VHHs fused together to different tags

From: Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies

 

A10 mg/L

C8 mg/L

G3 mg/L

Myc per IMAC

1-5

1-5

<1

Myc per SEC

1-2

<1

<0.5

SNAP cyt IMAC

15-20

20-25

10-15

SNAP cyt SEC

15-20

20-25

10-15

SORT-Tag cyt IMAC

6-8

4-7

4-7

SORT-Tag cyt SEC

4-6

3-5

3-5

SORT-Tag per IMAC

<0.1

<0.1

<0.1

Fc cyt IMAC

25-30

10-15

15-20

Fc cyt SEC

25-30

10-15

10-15

  1. The recombinant antibodies were cloned in vectors that promote either periplasmic (per) or cytoplasmic (cyt) accumulation and folding, expressed in bacteria, and finally purified by metal affinity chromatography (IMAC) followed by gel filtration (Size Exclusion Chomatography - SEC). The yields are expressed in mg/L of culture and indicate the range obtained in at least three independent purification procedures.
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Microbial Cell Factories

ISSN: 1475-2859

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