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Table 2 End point HPLC analysis of substrates and products from fermentations shown in Figure 3

From: Fermentation of oxidized hexose derivatives by Clostridium acetobutylicum

 

Substrate used (mM)

Acetate (mM)

Butyrate (mM)

Ac:Bu

ATP/Substrate

Portion of C in products

Glucose Static

34.4(±0.1)

13.4(±0.4)

19.1(±0.3)

0.70

3.41

0.751

Glucose Agitated

34.9(±0.1)

15.8(±0.3)

19(±0.3)

0.83

3.45

0.771

Gluconate Static

28.2(±0.3)

29.2(±1.8)

9(±0.7)

3.24

2.43

0.840

Gluconate Agitated

28.5(±0.6)

31.2(±2.1)

8.9(±0.7)

3.50

2.44

0.860

0.5% Galacturonate Static

15.9(±0.2)

25.3(±0.3)

0.4(±0.0)

70.3

2.65

0.817

0.5% Galacturonte Agitated

17.3(±0.1)

28.3(±0.0)

0.6(±0.1)

47.1

2.65

0.850

0.75% Galactuornate Static

24 (±0.1)

39.3(±0.2)

1.4(±0.0)

27.7

2.63

0.879

0.75% Galacturonate Agitated

24.8(±0.2)

41.3(±0.7)

1.5(±0.0)

27.5

2.63

0.895

  1. Measured values are bold and represent three biological replicates.
  2. Theoretical calculations are italicized.