High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities

Table 3 Analytic data of human FLAG-Δ108ST6Gal-I expressed under fed-batch conditions

Construct	Intensity of variant in ESMS [%]	MS analysis	Truncation	IgG1		Asialofetuin
Construct	Intensity of variant in ESMS [%]	MS analysis	Truncation	G2 + 1SA [%]	G2 + 2SA [%]	Fetuin [RFU/μ g]
FLAG-Δ108ST6Gal-I*	85	DYKDDDDKLQKIWKN…	Δ108	14	0	33
	10	NYLS…	Δ114
	5	YLS…	Δ115

ISSN: 1475-2859