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Figure 4 | Microbial Cell Factories

Figure 4

From: Influence of pH control in the formation of inclusion bodies during production of recombinant sphingomyelinase-D in Escherichia coli

Figure 4

Comparison of logarithmic cumulative volume (%) distributions of the rSMD IB sizes harvested at 1, 3, 5 and 20 h after induction under controlled pH 7.5 condition (A), and under uncontrolled pH strategy (B). Distribution of IB size was determined in a Particle Sizer (Zetasizer Nano). The graphs were cut at 1300 μm because the contribution to scattering by particles > 1300 μm in size was only approximately 3%, except in data from controlled pH cultures at 3 h post-induction (12%). Figures show the accumulated values ± standard deviation of data from experiments performed by triplicate.

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Microbial Cell Factories

ISSN: 1475-2859

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