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Table 3 Production of catechol in bioreactor cultures by E. coli 9923 derivatives

From: Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1

Culture conditions/Strain

Biomass (g DCW /L)

μ (h −1 )

q Glc (g Glc /g DCW h)

q Ant (g Ant /g DCW h)

q Cat (g Cat /g DCW h)

Anthranilate (g/L)

Catechol (g/L)

Y Ant/Glc (g Ant /g Glc )

Y Cat/Glc (g Cat /g Glc )

37°C, M9 +

       

30 g/L Yeast Extract

W31109923/pJLBaroG fbr tktA*

18.54 ± 0.19

0.18 ± 0.01

0.14 ± 0.05

0.02 ± 0.01

-

14.00 ± 0.07

-

0.20 ± 0.00

-

W3110 9923/pJLBaroG fbr tktA/pTrc-ant3

10.44 ± 1.14

0.17 ± 0.03

0.13 ± 0.02

0.02 ± 0.00

0.02 ± 0.01

2.86 ± 0.09

2.51 ± 0.21

0.09 ± 0.02

0. 07 ± 0.02

37°C, M9

       

W3110 9923/pJLBaroG fbr tktA

7.66 ± 0.64

0.17 ± 0.01

0.12 ± 0.03

0.02 ± 0.00

-

8.18 ± 0.08

-

0.17 ± 0.01

-

W3110 9923/pJLBaroG fbr tktA/pTrc-ant3

7.13 ± 0.37

0.16 ± 0.02

0.11 ± 0.01

0.02 ± 0.00

0.02 ± 0.00

2.12 ± 0.08

2.81 ± 0.15

0.09 ± 0.01

0. 08 ± 0.02

32°C, M9

       

W3110 9923/pJLBaroG fbr tktA

7.51 ± 0.44

0.14 ± 0.01

0.09 ± 0.01

0.02 ± 0.00

-

8.61 ± 0.06

-

0.18 ± 0.02

-

W3110 9923/pJLBaroG fbr tktA/pTrc-ant3

6.92 ± 0.27

0.13 ± 0.01

0.07 ± 0.02

0.02 ± 0.00

0.02 ± 0.01

2.89 ± 0.15

4.47 ± 0.16

0.10 ± 0.02

0. 16 ± 0.02

28°C, M9

       

W3110 9923/pJLBaroG fbr tktA

7.37 ± 0.77

0.10 ± 0.01

0.08 ± 0.01

0.01 ± 0.00

-

6.28 ± 0.10

-

0.11 ± 0.01

-

W3110 9923/pJLBaroG fbr tktA/pTrc-ant3

6.75 ± 0.34

0.12 ± 0.01

0.06 ± 0.00

0.01 ± 0.00

0.01 ± 0.01

2.21 ± 0.14

1.83 ± 0.13

0.09 ± 0.02

0. 06 ± 0.01

  1. *Results from Balderas et al. [8].