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Table 1 Escherichia coli strains and plasmids used in this work

From: Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1

Strain/Plasmid

Characteristics

Reference

Strains

  

W3110 trpD9923

W3110 [F− Δ− INV (rrnD-rrnE) 1] tryptophan auxotroph, randomly mutagenized by treatment with ultraviolet radiation.

[13]

W3110 trpD9923 PTS−

As W3110 trpD9923 but Δ ptsHI, crr::kmR, glucose−

[8]

Plasmids

  

pJLBaroG fbr tktA

Containing the tktA gene with its native promoter. aroG fbr expressed from the lacUV5 promoter, lacI q and tet genes, tetracycline resistance, pACYC184 replication origin.

[8]

pTrc-ant3

Carries the antABC genes from Pseudomonas aeruginosa PAO1 under the control of the trc-derived promoter. Ampicillin resistance.

This work

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Microbial Cell Factories

ISSN: 1475-2859

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