Table 2 Primers used in this study
From: MEP pathway-mediated isopentenol production in metabolically engineered Escherichia coli
Name | Sequence (5′-3′) | Function |
|---|---|---|
Dxs-F | CGTCGGATCCATGAGTTTTGATATTGCCA | For PCR amplification of dxs |
Dxs-R | CGGGAATTCTTATGCCAGCCAGGCCTTG | |
IspG-F | CACGAGCTCAGGAGATATACCATGCATAACCAGGCTCCAAT | For PCR amplification of ispG |
IspG-R | CGTGAGCTCTTATTTTTCAACCTGCTGAACGT | |
Eddp1-KF | TCTGCGCTTATCCTTTATGGTTATTTTACCGGTAACATGACATATGAATATCCTCCTTAGT | For PCR amplification of eddp1 replacement cassette |
Eddp1-KF | CAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATTGTTTATTCCTCCTTACATTATACGAGCCGGATGATTAATTGTCAAGTGTAGGCTGGAGCTGCTTCG | |
TrcIN-F | GTGGCGATGATTACCCGTGA | For verification of eddp1 replacement with pTrc |
TrcIN-R | GGTTACCGCATGCCAACTGC | |
Pgi-KF | CTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATCATATGAATATCCTCCTTAGT | For PCR amplification of pgi disruption cassette |
Pgi-KR | TACCGTTACGGTCAACATACTTACCGTTGGACTCCATATTGTGTAGGCTGGAGCTGCTTCG | |
Pgi-F | TACTCCAAAAACCGCATCAC | For verification of pgi disruption |
Pgi-R | CGAAGAAGTTAGACAGCAGT |