Skip to main content

Table 1 Strains and plasmids used in this work

From: MEP pathway-mediated isopentenol production in metabolically engineered Escherichia coli

Plasmid/strain Function/characteristic Resource/reference
pACYC-yhfR yhfR expression vector This work
pACYC-nudF nudF expression vector This work
pACYC-ispG-nudF ispG and nudF co-expression vector This work
pACYC-dxs-ispG-nudF dxs, ispG and nudF co-expression vector ATCC No. 27325
W3110 E. coli W3110 F, λ IN (rrnD-rrnE)1 ATCC No. 27325
Δpgi E. coli W3110 Δpgi This work
Δpgi Δeddp1::pTrc E. coli W3110 Δpgi Δeddp1::pTrc This work
EWIP1 E. coli W3110 (DE3)/pACYC- yhfR This work
EWIP2 E. coli W3110 (DE3)/pACYC-nudF This work
EWIP3 E. coli W3110 (DE3)/pACYC-ispG-nudF This work
EWIP4 E. coli W3110 (DE3)/pACYC-dxs-ispG-nudF This work
EWIP5 E. coli W3110 (DE3) Δpgi/pACYC-dxs-ispG-nudF This work
EWIP6 E. coli W3110 (DE3) Δpgi Δeddp1::pTrc/pACYC-dxs-ispG-nudF This work