Table 1 Strains and plasmids used in this work

pACYC-yhfR

yhfR expression vector

This work

pACYC-nudF

nudF expression vector

This work

pACYC-ispG-nudF

ispG and nudF co-expression vector

This work

pACYC-dxs-ispG-nudF

dxs, ispG and nudF co-expression vector

ATCC No. 27325

W3110

E. coli W3110 F⁻, λ⁻ IN (rrnD-rrnE)1

ATCC No. 27325

Δpgi

E. coli W3110 Δpgi

This work

Δpgi Δeddp1::pTrc

E. coli W3110 Δpgi Δeddp1::pTrc

This work

EWIP1

E. coli W3110 (DE3)/pACYC- yhfR

This work

EWIP2

E. coli W3110 (DE3)/pACYC-nudF

This work

EWIP3

E. coli W3110 (DE3)/pACYC-ispG-nudF

This work

EWIP4

E. coli W3110 (DE3)/pACYC-dxs-ispG-nudF

This work

EWIP5

E. coli W3110 (DE3) Δpgi/pACYC-dxs-ispG-nudF

This work

EWIP6

E. coli W3110 (DE3) Δpgi Δeddp1::pTrc/pACYC-dxs-ispG-nudF

This work