NoV VLPs production. A) On-line methanol control performance. Induction phase starts 24 h after inoculation. The arrow indicates beginning of methanol addition. B) Every 24 h aliquots were taken from the fermentation media. Cells and supernatant were separated by centrifugation and 10 μl of each supernatant sample was loaded into a SDS-PAGE gel. C) Protein concentration was determined for each aliquot, taken every 24 h, by Bradford.