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Figure 1 | Microbial Cell Factories

Figure 1

From: Secreted production of assembled Norovirus virus-like particles from Pichia pastoris

Figure 1

NoV VLPs production. A) On-line methanol control performance. Induction phase starts 24 h after inoculation. The arrow indicates beginning of methanol addition. B) Every 24 h aliquots were taken from the fermentation media. Cells and supernatant were separated by centrifugation and 10 μl of each supernatant sample was loaded into a SDS-PAGE gel. C) Protein concentration was determined for each aliquot, taken every 24 h, by Bradford.

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