Table 2 Primers used in RT-PCR analysis
Primer | Forward | Reverse | Amplicon size (bp) | Amplicon dissociation temperature (°C) |
|---|---|---|---|---|
hrdB | GATGCTCTTCCTGGACCTGA | CTTGTTGATCACCTCGACCA | 193 | 87.9 |
phoP | GACAGCGAGATCGACAAGGT | AGCAGCTCGAACTCCTTGAG | 239 | 91.6 |
phoR | ACCGTCGAGATAAGCGTCAG | CTTGCTCCATACCGTGACCT | 186 | 90.3 |
pstS | AGAACTCGCAGCTCCAGAAG | TCGTAGGTCACCAGGACGAT | 187 | 89.6 |
ppK | GACGTCACCGAGAACCTCAT | CAGGCGGTAGATCTCGTTGT | 159 | 88.9 |
mlbA | GACTTCCGAGACCGAGGAC | GTGCACAGCGACCAAGCTC | 96 | 86.3 |
mlbB | GCTGGTTCTTCATCCGCTAC | CTCGTACTCGGGGTCGTACT | 179 | 90.6 |
mlbD | AACCCGGTGATGTGGCGCAAGC | CCGGCCGAGGCATGGTCAGTC | 148 | 90.7 |
mlbE | CACGCTGGCGCTACTTCACCG | TCGAGGCGGCGTTGGAGAGCT | 129 | 90 |
mlbY | CGCCCTGCTCGTGTACGCCGT | GAACGCGGCGATCAGCAGTCC | 121 | 89.4 |