Figure 5From: Improved productivity of poly (4-hydroxybutyrate) (P4HB) in recombinant Escherichia coli using glycerol as the growth substrate with fed-batch culture High cell density cultivation with linear feeding mode of E . coli JM109 (pKSSE5.3). The feeding solution contained 200gL-1 acetic acid and 200gL-1 glycerol for 65h and then 100gL-1 acetic acid and 200gL-1 glycerol. Panel A: feeding rate of 1mLh-1; B: feeding rate of 2mLh-1. Arrows represent the start of feeding. The data are the average numbers of duplicates.Back to article page