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Figure 8 | Microbial Cell Factories

Figure 8

From: Deletion of the gene encoding the reductase component of 3-ketosteroid 9α-hydroxylase in Rhodococcus equi USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione

Figure 8

Macrophage infection assay with E. coli Top10F', R. equi USA-18, and R. equi USA-18ΔB8. Macrophages derived from the human THP-1 cell line were infected with the indicated bacteria at a multiplicity of infection of 10 as described in Materials and methods. The number of intracellular bacteria within the macrophages was determined by plate counting at 4, 24, and 96 h post-infection.

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