Skip to main content
Figure 4 | Microbial Cell Factories

Figure 4

From: Deletion of the gene encoding the reductase component of 3-ketosteroid 9α-hydroxylase in Rhodococcus equi USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione

Figure 4

Analysis of cholesterol metabolite by HPLC. R. equi USA-18ΔB8 was cultivated in medium including 0.2% cholesterol and 0.2% Tween 20 at 37°C, 200 rpm. The broth, harvested at the indicated time, was extracted with ethyl acetate and the compounds within were analyzed by HPLC using a reverse-phase column as described in Materials and methods.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us