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Figure 2 | Microbial Cell Factories

Figure 2

From: Deletion of the gene encoding the reductase component of 3-ketosteroid 9α-hydroxylase in Rhodococcus equi USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione

Figure 2

Confirmation of the replacement of the putative chromosomal KshB with Kan by PCR. Primer 10 specifically targets at Kan, while primer 13 and 14 at KshB. Primer 12 matches the downstream region of the putative KshB. The nucleotide sequences of the primers are shown in Table 1.

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Microbial Cell Factories

ISSN: 1475-2859

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