Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

Table 2 Functional recombinant protein is present in the pre-induction phases of P. pastoris cultures when cultivated in bioreactors, but not in shake-flasks

Protein	Bioreactor		Shake-flask
	Pre-induction expression in glycerol-grown cells?	Pre-induction expression in glucose-grown cells?	Pre-induction expression in glycerol-grown cells?	Pre-induction expression in glucose-grown cells?
*Integral membrane proteins*
hA_2aR	Y (Figures 1 and 2)	Y (Figure 4)	N [18]	-
CD81	-	-	N (Additional file 1: Figure S1)	-
CD82	-	-	N (Additional file 1: Figure S1)	N (Additional file 1: Figure S1)
Claudin-1	-	-	N (Additional file 1: Figure S1)	N (Additional file 1: Figure S1)
*Peripheral membrane protein*
hCGRP-RCP-GFP	Y (Table S1)	-	-	-
*Soluble proteins*
GFP	Y (Figures 3 and 6A)	Y (Figures 5 and 6B)	N (Additional file 1: Table S2)	N (Additional file 1: Table S2)
HRP	-	-	N (Additional file 1: Table S3)	-

The presence (Y) or absence (N) of recombinant protein was determined in bioreactor and shake-flask cultivations for glycerol- and glucose-grown cells as indicated; the evidence supporting these designations is given in parentheses and refers to figures within the text or in supplementary files; “–” indicates that the experiment was not done.

ISSN: 1475-2859