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Table 2 Functional recombinant protein is present in the pre-induction phases of P. pastoris cultures when cultivated in bioreactors, but not in shake-flasks

From: Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

Protein Bioreactor Shake-flask
  Pre-induction expression in glycerol-grown cells? Pre-induction expression in glucose-grown cells? Pre-induction expression in glycerol-grown cells? Pre-induction expression in glucose-grown cells?
Integral membrane proteins
hA2aR Y (Figures 1 and 2) Y (Figure 4) N [18] -
CD81 - - N (Additional file 1: Figure S1) -
CD82 - - N (Additional file 1: Figure S1) N (Additional file 1: Figure S1)
Claudin-1 - - N (Additional file 1: Figure S1) N (Additional file 1: Figure S1)
Peripheral membrane protein
hCGRP-RCP-GFP Y (Table S1) - - -
Soluble proteins
GFP Y (Figures 3 and 6A) Y (Figures 5 and 6B) N (Additional file 1: Table S2) N (Additional file 1: Table S2)
HRP - - N (Additional file 1: Table S3) -
  1. The presence (Y) or absence (N) of recombinant protein was determined in bioreactor and shake-flask cultivations for glycerol- and glucose-grown cells as indicated; the evidence supporting these designations is given in parentheses and refers to figures within the text or in supplementary files; “–” indicates that the experiment was not done.