Table 1 Pre-induction yields of hA 2a R are maintained at pilot scale even in the presence of cytotoxic methanol concentrations
| Â | Phase | Age (h) | Residual glycerol (g L-1) | Residual methanol (g L-1) | DCW (g L-1) | Total membrane protein yield in 1 L culture (mg) | Bmaxestimate (pmol mg-1) | Total hA2aR yield in 1 L culture (pmol) | Specific yield (pmol g-1) |
|---|---|---|---|---|---|---|---|---|---|
Low methanol cultivation | IIIA | 42.2 | 0 (0) | 0 (0) | 41.91 (0.6) | 764.4 | 4.4 (0.1) | 3363.6 | 80.3 |
IIIB | 44.4 | 1.1 (0.2) | 0.5 (0.1) | 42.10 (0.4) | 2791.1 | 7.1 (0.6) | 19816.9 | 470.7 | |
IV | 66.4 | 0 (0) | 1.0 (0.1) | 50.53 (0.5) | 4084.4 | 4.5 (0.2) | 18380.0 | 363.7 | |
IV | 89.0 | 0 (0) | 0 (0) | 71.82 (0.2) | 7704.4 | 5.0 (0.2) | 38522.2 | 536.4 | |
High methanol cultivation | IIIA | 42.2 | 2.9 (0.1) | 0 (0) | 46.71 (0.5) | 813.3 | 4.4 (0.2) | 3578.7 | 76.6 |
IIIB | 44.4 | 4.2 (0.2) | 2.1 (0.2) | 46.66 (0.7) | 3060.0 | 4.0 (0.0) | 12240.0 | 262.3 | |
IV | 66.4 | 1.1 (0.2) | 39.1 (9.0) | 45.76 (0.5) | 3277.8 | 3.8 (0.1) | 12455.5 | 272.2 | |
V | 88.9 | 0 (0) | 84.7 (0.5) | 37.64 (0.8) | 1797.8 | 3.1 (0.1) | 5573.1 | 148.1 |