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Figure 4 | Microbial Cell Factories

Figure 4

From: Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

Figure 4

Recombinant hA 2a R is produced in the pre-induction phases of 1 L glucose-grown P. pastoris bioreactor cultivations. hA2aR binding activity (pmol mg-1; red circles) was measured in the culture supernatant in all the phases of a 1L glucose-grown P. pastoris cultivation. The residual glucose concentration (mM; white inverted triangles) ranged from 0.01-0.04 mM during the cultivation was not consumed completely by the cells. The residual methanol (g L-1; black squares) ranged from 0.20 to 99.76 g L-1. The DCW (g L-1; white circles) for this cultivation reached a maximum of 21 g L-1. All measurements were made in triplicate and error bars represent the SEM.

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