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Figure 3 | Microbial Cell Factories

Figure 3

From: Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

Figure 3

Recombinant GFP is produced in the pre-induction phases of 1 L glycerol-grown P. pastoris bioreactor cultivations. GFP yield (mg L-1; green squares) was measured in the culture supernatant in all phases of duplicate 1 L glycerol-grown P. pastoris cultures. The residual glycerol concentration (g L-1; white triangles) was at its highest during phase I, eventually dropping to zero in phase II. The DCW (white circles) for this cultivation reached a maximum of 175 g L-1. Residual methanol (g L-1; black squares) ranged from 3.01 to 8.52 g L-1. All measurements were made in triplicate and error bars represent the SEM.

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