Figure 1

hA _2a R binding activity is present in all phases of 1 L glycerol-grown P. pastoris bioreactor cultivations. Duplicate 1 L bioprocesses were analysed for hA_2aR binding activity (pmol mg^-1; red circles), DCW (g L^-1; white circles), residual glycerol concentration (g L^-1; white triangles) and residual methanol concentration (g L^-1; black squares). hA_2aR binding activity was measured in all the phases, including pre-induction phases I and II. The residual glycerol concentration was at its highest during phase II, dropping to zero in phase II and for the duration of the cultivation. DCW increased during phases I and II and plateaued during phases III and IV. Residual methanol ranged from 1.25 – 7.66 g L^-1 during the induction phase. Measurements were made in triplicate for each culture. The error bars represent standard error of the mean (SEM).