Table 1 Assaying the in vitro decarboxylase activity of FDC1 against a pool of structurally-related, phenylacrylic acid substrates
From: Rational and combinatorial approaches to engineering styrene production by Saccharomyces cerevisiae
Compound | Induced activity | mU mg −1 total protein |
|---|---|---|
trans-cinnamate | + | 0.46 ± 0.02 |
p-coumarate | + | 0.39 ± 0.02 |
ferulate | + | 0.21 ± 0.03 |
phenylalanine | - | N.D. |
none (control) | - | N.D. |