Figure 2
From: Rational and combinatorial approaches to engineering styrene production by Saccharomyces cerevisiae
Evolution of phenylalanine overproducing mutants of S. cerevisiae . Mutants were evolved through the use of EMS mutagenesis and high-throughput selection on solid agar plates using m-fluoro-DL-phenylalanine as anti-metabolite. 2-Phenylethanol (gray) and 2-phenylacetate (hashed) production by isolated mutants was determined after 48Â h of growth in SD media by measuring the concentration of 2-phenylethanol and 2-phenylacetate in the supernatant. Error bars reported at one standard deviation from triplicate experiments.